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122 1260 Nanoflow Pump User Manual
8 Test Functions and Calibration
Leak Test
Third plateau negative or unstable and at least one other plateau positive
Probable cause Suggested actions
1 Air in channel A or new seals not yet seated.
Flush channel A thoroughly with isopropanol
under pressure (use restriction capillary).
2 Loose active inlet valve in channel A.
Tighten the active inlet valve in channel A
(14 mm wrench). Do not overtighten!
3 Loose pump head screws in channel A.
Ensure the pump head screws in channel A are
tight.
4 Loose outlet valve in channel A.
Ensure the sieve in the outlet valve is installed
correctly. Tighten the outlet valve.
5 Leaking seal or scratched piston in channel
A1.
Exchange the pump seals in channel A. Check
the pistons for scratches. Exchange if scratched.
6 Defective active inlet valve in channel A.
Exchange the active inlet valve in channel A.
- Agilent 1260 Infinity 1
- Nanoflow Pump 1
- Contents 3
- Figure 1 Overview of the Pump 9
- Hydraulic Path Overview 10
- Introduction to the Pump 11
- Early Maintenance Feetback 16
- Instrument Layout 17
- Electrical Connections 18
- Rear View of the Module 19
- Interfaces 20
- Overview Interfaces 22
- Analog Signal Output 23
- APG Remote 24
- Special Interfaces 25
- Special Settings 31
- Forced Cold Start 32
- Site Requirements 34
- Power Cords 35
- Bench Space 36
- Condensation 36
- Physical Specifications 37
- Performance Specifications 38
- Installing the Module 41
- Unpacking the Module 42
- One Stack Configuration 44
- 3 Installing the Module 46
- Two Stack Configuration 47
- Installing the Pump 49
- Connecting Modules 52
- Connecting a Vacuum Degasser 52
- Flow Connections 54
- Using the Pump 61
- 4 Using the Pump 62
- Pump Issues 62
- Fused Silica Capillary Issues 63
- Algae Growth in HPLC Systems 65
- Method Parameters 68
- Test Results and Evaluation 70
- Optimizing Performance 71
- 5 Optimizing Performance 72
- Choosing the Right Pump Seals 73
- The Standard Filter 75
- Status Indicators 80
- Error Messages 80
- Test Functions 80
- System Pressure Test 80
- Leak Rate Test 81
- Module Status Indicator 83
- User Interfaces 84
- Agilent Lab Advisor Software 85
- Error Information 87
- 7 Error Information 88
- What Are Error Messages 89
- General Error Messages 90
- Remote Timeout 91
- Synchronization Lost 91
- Leak Sensor Short 92
- Leak Sensor Open 92
- Compensation Sensor Open 93
- Compensation Sensor Short 93
- Fan Failed 94
- Module Error Messages 95
- Pressure Below Lower Limit 96
- Pressure Signal Missing 96
- Valve Failed 97
- Missing Pressure Reading 98
- Pump Configuration 98
- Valve Fuse 99
- Inlet-Valve Fuse 99
- Temperature Out of Range 100
- Temperature Limit Exceeded 100
- Motor-Drive Power 101
- Encoder Missing 102
- Inlet-Valve Missing 102
- Leak Sensor Missing 103
- Flow Sensor Missing 103
- Servo Restart Failed 104
- Pump Head Missing 105
- Index Limit 105
- Index Adjustment 106
- Index Missing 106
- Stroke Length 107
- Initialization Failed 107
- Wait Timeout 108
- Electronic fuse of SSV 108
- Agilent Technologies 109
- Micro Mode Pressure Test 110
- If the pressure test fails 111
- Normal Mode Pressure Test 112
- Positioning the Blank Nut 113
- Running the Pressure Test 114
- Evaluating the Results 115
- Leak Test 116
- Running the Leak Test 118
- All plateaus negative 120
- EMPV Test 124
- EMPV Cleaning 125
- Maintenance 127
- Introduction to Maintenance 128
- Warnings and Cautions 129
- Overview of Maintenance 130
- Cleaning the Module 132
- EMF Counters 134
- Using the EMF Counters 135
- 9 Maintenance 136
- Tools required Wrench 14 mm 139
- 6Xi^kZ^caZikVakZ 141
- HdakZci^caZiijWZ 141
- KVakZXVe^aaVgn 143
- DjiaZiWVaakVakZ 143
- 8dccZXi^c\ijWZh 145
- HdakZciijWZh 145
- HdakZcihZaZXi^dc 145
- 8]VbWZgh 149
- Seal Wear-in Procedure 150
- Exchanging the Pistons 151
- Exchanging the Flow Sensor 153
- Replacing Module Firmware 157
- Item p/n Description 162
- Hydraulic Path 164
- Figure 27 Hydraulic Path 165
- Pump-Head Assembly 166
- Figure 28 Pump-Head Assembly 167
- Flow Sensor Assembly 168
- Nanoflow Pump Accessory Kit 169
- Identifying Cables 171
- Cable Overview 172
- Analog Cables 174
- Remote Cables 176
- 11 Identifying Cables 178
- BCD Cables 179
- CAN/LAN Cables 181
- External Contact Cable 182
- Agilent Module to PC 183
- Appendix 185
- General Safety Information 186
- Safety Symbols 188
- Abstract 189
- Batteries Information 190
- Radio Interference 191
- Sound Emission 192
- Solvent Information 193
- 12 Appendix 194
- *G2226-90012* 200
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