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72 1260 Nanoflow Pump User Manual
5 Optimizing Performance
Hints for the Micro Vacuum Degasser
Hints for the Micro Vacuum Degasser
If you are using the vacuum degasser for the first time, if the vacuum degasser
was switched off for any length of time (for example, overnight), or if the
vacuum degasser lines are empty, you should prime the vacuum degasser
before running an analysis.
The vacuum degasser can be primed by pumping solvent with the 1260
Nanoflow Pump at high flow rate (2.5 mL/min). Priming the degasser is
recommended, when:
• vacuum degasser is used for the first time, or vacuum chambers are empty,
• changing to solvent that are immiscible with the solvent currently in the
vacuum chambers,
• the pump was turned OFF for a length of time (for example over night) and
volatile solvent mixtures are used.
For more information see Agilent 1260 Infinity (G1379B) Micro Degasser User
Manual (p/n G1379-90013).
- Agilent 1260 Infinity 1
- Nanoflow Pump 1
- Contents 3
- Figure 1 Overview of the Pump 9
- Hydraulic Path Overview 10
- Introduction to the Pump 11
- Early Maintenance Feetback 16
- Instrument Layout 17
- Electrical Connections 18
- Rear View of the Module 19
- Interfaces 20
- Overview Interfaces 22
- Analog Signal Output 23
- APG Remote 24
- Special Interfaces 25
- Special Settings 31
- Forced Cold Start 32
- Site Requirements 34
- Power Cords 35
- Bench Space 36
- Condensation 36
- Physical Specifications 37
- Performance Specifications 38
- Installing the Module 41
- Unpacking the Module 42
- One Stack Configuration 44
- 3 Installing the Module 46
- Two Stack Configuration 47
- Installing the Pump 49
- Connecting Modules 52
- Connecting a Vacuum Degasser 52
- Flow Connections 54
- Using the Pump 61
- 4 Using the Pump 62
- Pump Issues 62
- Fused Silica Capillary Issues 63
- Algae Growth in HPLC Systems 65
- Method Parameters 68
- Test Results and Evaluation 70
- Optimizing Performance 71
- 5 Optimizing Performance 72
- Choosing the Right Pump Seals 73
- The Standard Filter 75
- Status Indicators 80
- Error Messages 80
- Test Functions 80
- System Pressure Test 80
- Leak Rate Test 81
- Module Status Indicator 83
- User Interfaces 84
- Agilent Lab Advisor Software 85
- Error Information 87
- 7 Error Information 88
- What Are Error Messages 89
- General Error Messages 90
- Remote Timeout 91
- Synchronization Lost 91
- Leak Sensor Short 92
- Leak Sensor Open 92
- Compensation Sensor Open 93
- Compensation Sensor Short 93
- Fan Failed 94
- Module Error Messages 95
- Pressure Below Lower Limit 96
- Pressure Signal Missing 96
- Valve Failed 97
- Missing Pressure Reading 98
- Pump Configuration 98
- Valve Fuse 99
- Inlet-Valve Fuse 99
- Temperature Out of Range 100
- Temperature Limit Exceeded 100
- Motor-Drive Power 101
- Encoder Missing 102
- Inlet-Valve Missing 102
- Leak Sensor Missing 103
- Flow Sensor Missing 103
- Servo Restart Failed 104
- Pump Head Missing 105
- Index Limit 105
- Index Adjustment 106
- Index Missing 106
- Stroke Length 107
- Initialization Failed 107
- Wait Timeout 108
- Electronic fuse of SSV 108
- Agilent Technologies 109
- Micro Mode Pressure Test 110
- If the pressure test fails 111
- Normal Mode Pressure Test 112
- Positioning the Blank Nut 113
- Running the Pressure Test 114
- Evaluating the Results 115
- Leak Test 116
- Running the Leak Test 118
- All plateaus negative 120
- EMPV Test 124
- EMPV Cleaning 125
- Maintenance 127
- Introduction to Maintenance 128
- Warnings and Cautions 129
- Overview of Maintenance 130
- Cleaning the Module 132
- EMF Counters 134
- Using the EMF Counters 135
- 9 Maintenance 136
- Tools required Wrench 14 mm 139
- 6Xi^kZ^caZikVakZ 141
- HdakZci^caZiijWZ 141
- KVakZXVe^aaVgn 143
- DjiaZiWVaakVakZ 143
- 8dccZXi^c\ijWZh 145
- HdakZciijWZh 145
- HdakZcihZaZXi^dc 145
- 8]VbWZgh 149
- Seal Wear-in Procedure 150
- Exchanging the Pistons 151
- Exchanging the Flow Sensor 153
- Replacing Module Firmware 157
- Item p/n Description 162
- Hydraulic Path 164
- Figure 27 Hydraulic Path 165
- Pump-Head Assembly 166
- Figure 28 Pump-Head Assembly 167
- Flow Sensor Assembly 168
- Nanoflow Pump Accessory Kit 169
- Identifying Cables 171
- Cable Overview 172
- Analog Cables 174
- Remote Cables 176
- 11 Identifying Cables 178
- BCD Cables 179
- CAN/LAN Cables 181
- External Contact Cable 182
- Agilent Module to PC 183
- Appendix 185
- General Safety Information 186
- Safety Symbols 188
- Abstract 189
- Batteries Information 190
- Radio Interference 191
- Sound Emission 192
- Solvent Information 193
- 12 Appendix 194
- *G2226-90012* 200
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