Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 10)

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Part # 15035786 Rev. D

Introduction 70

Rapid Run Sequencing Workflow 72

Run Types for Rapid Run Chemistry 73

Pre-Run Volume Check 75

Enter Run Parameters 76

Load and Prime Reagents 81

Load a Flow Cell 90

Monitor the Run 96

Chapter 5 Post-Run Procedures 97

Introduction 98

Unload and Weigh Reagents 99

Perform a Maintenance Wash 100

Perform a Water Wash 104

Switch Sequencing Modes 106

Idle the Instrument 108

Shut Down the Instrument 109

Chapter 6 Real-Time Analysis 111

Introduction 112

Real-Time Analysis Overview 113

Monitor Run Metrics 115

Real-Time Analysis Workflow 117

Sequencing Output Files 121

Output Folder Structure 123

Tile Numbering 125

Thumbnail Images 126

Chapter 7 Troubleshooting 127

Introduction 128

Possible Run Setup Problems 129

Stagger Runs on Flow Cell A and Flow Cell B 130

Perform a Fluidics Check 131

BaseSpace is Unavailable 132

Stop and Resume a Run 133

Pause a Run 136

Primer Rehybridization 137

Index 139

Technical Assistance 143

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