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Perform a HiSeq v4 Run

Part # 15035786 Rev. D

Introduction

To perform a HiSeq v4 run on the HiSeq 2500, prepare all reagents for the run and then

follow the software prompts to set up the run. Run setup steps include entering run

parameters, loading and priming reagents, loading the flow cell, and performing a fluidics

check.

Visit the HiSeq 2500 specifications page on the Illumina website for information about run

duration and other performance specifications.

HiSeq v4 Sequencing Consumables

HiSeq v4 Kit Name Description

HiSeq SBS Kit v4 Contains SBSreagents used on the HiSeq 2500.

HiSeq PE Cluster Kit v4

HiSeq SRCluster Kit v4

Contains clustering reagents used on the cBot and indexing

reagents used on the HiSeq 2500.

The PE version of the cluster kit includes paired-end reagents

used on the HiSeq 2500.

Each cluster kit includes an accessories kit that contains flow cell

gasket replacements and funnel caps for SBSreagent bottles.

Reagent Preparation Steps

Before setting up the run, prepare SBS reagents, indexing reagents, and paired-end reagents,

if applicable.

} For SBS reagent preparation, see the HiSeq SBS Kit v4 Reference Guide (part # 15050108).

} For indexing and paired-end reagent preparation, see the HiSeq Cluster Kit v4 Reference

Guide (part # 15050104).

Prepare all reagents before setting up the run. When prompted by the control software, load

all reagents. When using HiSeqv4 chemistry, there is no need to return to the instrument

during the run to load reagents.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 28