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Perform a HiSeq v4 Run

Part # 15035786 Rev. D

Load and Prime Reagents

After entering run parameters, load SBS, indexing, and paired-end reagents for the run, and

then prime reagents through the fluidics system. The software guides you through these

steps in a series of screens on the Pre-Run Setup tab.

Illumina-Supplied Consumables

} 8 funnel caps

User-Supplied Consumables

} 250ml bottle (Corning, catalog#430776)

} 15 ml conical tubes (Corning, catalog # 430052)

} Laboratory-grade water

NOTE

To prepare for the post-run rinse at the end of a sequencing run, load 25 ml PW1 or

laboratory-grade water in position 2.

The post-run rinse does not replace the post-run instrument wash.

Load SBS Reagents

1 Invert each bottle several times to make sure that the reagents are mixed thoroughly.

2 Remove the cap from each reagent bottle and replace it with a funnel cap.

CAUTION

After handling the bottle of CRM, discard your gloves and replace them with a new pair.

3 Record the weight of each reagent on the lab tracking form.

NOTE

Weighing reagents before and after a sequencing run confirms proper reagent delivery.

4 Open the reagent compartment door.

5 Raise the sippers for the sequencing reagent rack using the following motion:

a Pull the handle towards you and then raise the handle.

b Release the sipper handle into the slot on the top end of the groove. Make sure that

the sipper handle rests securely in the slot.

6 Slide the reagent rack out of the reagent compartment.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 36