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Load and Prime Reagents

HiSeq 2500 System User Guide

Load and Prime Reagents

After entering run parameters, load SBS and indexing reagents for the run, and then prime

reagents through the fluidics system. The software guides you through these steps in a

series of screens on the Pre-Run Setup tab.

Illumina-Supplied Consumables

} Eight funnel caps

User-Supplied Consumables

} 250ml bottle (Corning, catalog#430776)

} 15 ml conical tubes (Corning, catalog # 430052)

} Laboratory-grade water

NOTE

To prepare for the post-run rinse at the end of a sequencing run, load 25 ml PW1 or

laboratory-grade water in position 2.

The post-run rinse does not replace the post-run instrument wash.

Load SBS Reagents

1 Remove the cap from each reagent bottle and replace it with a funnel cap.

CAUTION

After handling the bottle of CMR, discard your gloves and replace them with a new pair.

2 Record the weight of each reagent on the lab tracking form.

NOTE

Weighing reagents before and after a sequencing run confirms proper reagent delivery.

3 Open the reagent compartment door.

4 Raise the sippers for the sequencing reagent rack using the following motion:

a Pull the handle towards you and then raise the handle.

b Release the sipper handle into the slot on the top end of the groove. Make sure that

the sipper handle rests securely in the slot.

5 Slide the reagent rack out of the reagent compartment.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 61