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Perform a Fluidics Check

HiSeq 2500 System User Guide

131

Perform a Fluidics Check

The Check button on the Welcome screen performs a fluidics check. Use this option during

instrument installation and when troubleshooting fluidics issues.

1 Load a used flow cell onto the instrument.

2 Load 8 SBSbottles with PW1 or laboratory-grade water, and load the bottles onto the

corresponding reagent rack. Load the rack onto the instrument.

3 Select Check on the Welcome screen.

4 Select solution5 (SB2) from the drop-down list. If you are performing a fluidics check

with a used flow cell, select solution2, which is water.

5 Enter the following default values:

• Volume: 250

• [For HiSeq v4 and TruSeq v3 modes] Aspirate Rate: 250

• [For Rapid Run mode] Aspirate Rate: 1500

• Dispense Rate: 2000

6 Select Pump. To pause the fluidics check, select Pause.

7 Visually inspect the flow cell for bubbles passing through the lanes and leaks near the

manifolds.

If excessive bubbles are present, check the manifold gaskets for obstructions, reduce the

aspirate rate to 100, and pump another 250µl of water to the flow cell.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 141