Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 111)

Perform a Maintenance Wash

HiSeq 2500 System User Guide

101

• Laboratory-grade water (750 ml)

• 10% Tween 20 (250 ml)

• ProClin 300 (1.5 ml)

These volumes result in approximately 2.5% Tween 20 and 0.15%ProClin 300 solution.

4 Place the carboy onto a stir plate and stir until the solution is thoroughly mixed.

5 Add 4 liters laboratory-grade water to the solution. These volumes result in

approximately 0.5% Tween 20 and 0.03% ProClin 300 wash solution.

6 Continue stirring until the solution is thoroughly mixed.

7 Set aside in a closed container at room temperature until you are ready to fill or

replenish reagent bottles and tubes with wash solution.

Tween 20 and ProClin 300 Wash

1 From the Welcome screen, select Wash | Maintenance.

2 [For high output modes] Select Yes to wash PEreagent positions when the sequencing

run included an indexing read or PEturn. Otherwise, select No. Select Next to proceed.

3 If you are using fresh wash solution, prepare the wash components as follows:

a Fill 8 SBSbottles with 250ml wash solution.

b Fill 10 PEtubes with 12ml wash solution.

c [For RapidRun mode] Fill Eppendorf tubes with 1.6 ml wash solution and load

them into the loading station.

If you are reusing wash solution, replenish the bottles and tubes saved from the

previous wash.

4 For fresh wash solution, assign each bottle and tube to a reagent rack position and

maintain those positions for each subsequent wash. Otherwise, the wash solution

might become contaminated with reagents that were present on the sippers.

5 Load the bottles and tubes onto the instrument in the assigned reagent rack position.

6 Select the Wash solution loaded and template loading station closed checkbox.

7 Select Next.

8 Remove the flow cell from the flow cell stage and set it aside until you are ready to

reload the same flow cell before starting the wash.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 111