Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 115)

Perform a Water Wash

HiSeq 2500 System User Guide

105

6 Remove the waste tubing for the appropriate flow cell from the waste container. Do not

include the waste tubing for the opposite flow cell, or the tubing from the condensation

pump.

7 [For high output modes] Bundle the waste tubing with parafilm, making sure to keep

all of the ends even. Place the bundled tubing ends into a 250ml bottle.

8 [For Rapid Run mode] Place the ends of tubes 4 and 5 into an empty container. Place

the end of all of the other tubing into a bottle of clean water to prevent air from being

introduced into the syringe pumps.

9 Select Next to start the water wash.

Positions Approximate Run

Time

8 SBS positions 20minutes

8 SBS positions and 10 paired-end positions 60minutes

[Rapid Run mode] 8 SBS positions, 10 paired-end positions, and 1 library

loading position

10minutes

10 When the wash is complete, measure the delivered volume and record it on the lab

tracking form.

Positions Delivered

Volume

8 SBS positions 32ml

8 SBS positions and 10 paired-end positions 72ml

[Rapid Run mode] 8 SBS positions, 10 paired-end positions, and 1 library

loading position

9.5 ml per lane

11 Unwrap the waste tubing and return the tubing to the waste bottle.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 115