Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 99)

Load and Prime Reagents

HiSeq 2500 System User Guide

CAUTION

Use water to confirm proper flow on a used flow cell only. Never use water to confirm

proper flow on a clustered flow cell.

2 Confirm the following default values:

• Volume: 250

• Aspirate Rate: 1500

• Dispense Rate: 2000

3 Select Pump.

4 Visually inspect the flow cell for bubbles passing through the lanes and leaks near the

manifolds.

If excessive bubbles are present, check the gaskets for obstructions, reduce the aspirate

rate to 1000, and pump another 250 µl of water to the flow cell. If problems persist,

remove the flow cell, repeat the cleaning steps, and reload the flow cell.

Position Tubing and Prime Reagents

1 Loosen and remove the waste tubes for the appropriate flow cell from the waste

container. Do not include the 8 tubes for the opposite flow cell or the tube for the

condensation pump.

2 Place tube 4 and tube 5 into separate 15 ml tubes.

3 Place tubes 1, 2, 3, 6, 7, and 8 into a bottle containing laboratory-grade water.

4 Select Next.

5 Select Start Prime to start priming. You can monitor the progress of the priming from

the Prime screen.

6 When priming is complete, measure the collected priming waste and confirm that the

volume is 2.5 ml ±10%, which is 500µl per reagent, per lane. Record the results on the

lab tracking form.

7 Return tubes 4 and 5 to the waste container before proceeding. Leave waste tubes 1, 2,

3, 6, 7, and 8 in the bottle containing laboratory-grade water.

8 Select Next.

1 2 ... 94 95 96 97 98 99 100 101 102 103 104 ... 153 154

Sin comentarios

Directed Electronics equalizer 2500 Guía de usuario Pagina 99