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HiSeq 2500 System User Guide

vii

Revision History

Part # Rev. Date

Description of Change

15035786

D November

2014

Updated Rapid Run mode workflow for compatibility with HiSeq Rapid v2

chemistry.

Replaced NaOH maintenance wash with Tween 20 and ProClin 300

maintenance wash including information about preparing, storing, and

disposing of maintenance wash solution.

Updated descriptions of maintenance wash and water wash to specify that

a water wash is required after a run.

Added workflow, input and output files, error handling, and quality

scoring descriptions to Real-Time Analysis chapter.

Updated VWR catalog # for alcohol wipes to 95041-714.

Updated URL for Safety Data Sheets (SDS) to

support.illumina.com/sds.html.

15035786 C April 2014

Updated software descriptions to HiSeq Control Software v2.2, which

includes the HiSeq v4 high output mode, removal of the control lane

option, default Q-score binning, and the option to use different indexing

schemes in each lane.

Added the HiSeq v4 workflow for use with HiSeq v4 chemistry.

Added calculation for total SBSpriming volume.

15035786 B November

2013

Removed reagent preparation instructions. For reagent prep instructions

including information about various sequencing primers, see the

documentation for the associated kit.

Replaced the following reagents:

• RMR for RMX

15035786 A October

2012

Initial release.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 7