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Enter Run Parameters

HiSeq 2500 System User Guide

A first base report is generated automatically for each run. Selecting this option opens

the first base report before proceeding with the run.

2 [Optional] From the Align to PhiX checkboxes, clear the checkbox for lanes that do not

contain PhiX.

By default, all lanes are selected for alignment by Real-Time Analysis software.

Alternatively, select lanes on the flow cell image to add or remove lanes for PhiX

alignment.

NOTE

A dedicated control lane is not required with HCS v2.2 and RTA v1.18. Therefore, the option

to assign a control lane is not available with this software configuration.

3 Select Next.

Recipe Screen

1 Select from the following Index Type options:

• No Index—Performs a non-indexed single-read or paired-end run.

• Single Index—Performs a single-read or paired-end run with 1 indexing read.

• Dual Index—Performs a single-read or paired-end run with 2 indexing reads.

• Custom—Performs a single-read or paired-end run with a custom number of cycles

for index reads.

2 If the Dual Index or Custom option is specified, select a Flow Cell Format, either Single

Read or Paired End.

3 Enter the number of cycles for Read 1 and Read 2, if applicable.

NOTE

The number of cycles performed in a read is 1 more cycle than the number of cycles

analyzed. For example, to perform 125 cycles for Read 1, enter 126.

For the Custom indexing option, enter the number of cycles for index reads. Read

lengths do not need to be identical.

4 Confirm the following default chemistry settings. These fields are auto-populated

depending on the selected index type option.

a SBS: HiSeq SBS Kit v4

b Index: HiSeq v4 Single Index or HiSeq v4 Dual Index

c PE turnaround: HiSeq PE Cluster Kit v4

5 [Optional] Select the Use Existing Recipe checkbox to use a custom recipe. Otherwise,

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Directed Electronics equalizer 2500 Guía de usuario Pagina 33