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Load and Prime Reagents

HiSeq 2500 System User Guide

Load and Prime Reagents

After entering run parameters, load SBS, clustering, indexing, and paired-end reagents for

the run, and then prime reagents through the fluidics system. The software guides you

through these steps in a series of screens on the Pre-Run Setup tab.

Illumina-Supplied Consumables

} Eight funnel caps

User-Supplied Consumables

} 250ml bottle (Corning, catalog#430776)

} 15 ml conical tubes (Corning, catalog # 430052)

} Laboratory-grade water

NOTE

To prepare for the post-run rinse at the end of a sequencing run, load 25 ml PW1 or

laboratory-grade water in position 2.

The post-run rinse does not replace the post-run instrument wash.

Load SBS and Clustering Reagents

Make sure that SBS reagents are ready to load onto the instrument.

Illumina-Supplied Consumables

} Eight funnel caps

User-Supplied Consumables

} One 250ml bottle (Corning, catalog#430776)

} One flip-top Eppendorf tube per flow cell (1.5 ml or 1.7 ml). Do not use tubes with

screw caps.

Procedure

1 Record the weight of each reagent on the lab tracking form.

2 Open the reagent compartment door.

3 Raise the sippers for the sequencing reagent rack using the following motion:

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Directed Electronics equalizer 2500 Guía de usuario Pagina 91