Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 92)

Perform a Rapid Run

Part # 15035786 Rev. D

a Pull the handle towards you and then raise the handle.

b Release the sipper handle into the slot on the top end of the groove. Make sure that

the sipper handle rests securely in the slot.

4 Slide the SBSreagent rack out of the reagent compartment.

5 Remove the cap from each reagent bottle and replace it with a funnel cap. Replace the

cap on the bottle of CRM last, and then replace your gloves.

6 Place each SBS reagent bottle onto the rack in the associated numbered position. Make

sure that the conical end of the bottle rests in the indentation on the base of the rack.

Position Reagent Description

1 IMT Incorporation Master Mix

2 PW1 25 ml PW1 or laboratory-grade water

3 USM Universal Scan Mix

4 PW1 25 ml PW1 or laboratory-grade water

5 USB Universal Sequencing Buffer

6 USB Universal Sequencing Buffer

7 CRM Cleavage Reagent Master Mix

8 CWM Cleavage Wash Mix

Table 12 HiSeq Rapid v2 SBS Reagent Positions

Position Reagent Description

1 IMM Incorporation Master Mix

2 PW1 25 ml PW1 or laboratory-grade water

3 SRM Scan Reagent Master Mix

4 PW1 25 ml PW1 or laboratory-grade water

5 USB Universal Sequencing Buffer

6 USB Universal Sequencing Buffer

7 CRM Cleavage Reagent Master Mix

8 PW1 25 ml PW1 or laboratory-grade water

Table 13 TruSeq Rapid (v1) SBS Reagent Positions

7 Load 25 ml PW1 or laboratory-grade water in the following positions:

• [For HiSeq Rapid SBSKit v2] Positions 2 and 4.

• [For TruSeq Rapid SBSKit] Positions 2, 4, and 8.

8 Slide the SBS rack into the reagent compartment, aligning the rack with the raised

guide on the floor of the compartment.

1 2 ... 87 88 89 90 91 92 93 94 95 96 97 ... 153 154

Sin comentarios

Directed Electronics equalizer 2500 Guía de usuario Pagina 92