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Enter Run Parameters

HiSeq 2500 System User Guide

4 Confirm the following chemistry settings. These fields are auto-populated depending

on the selected reagent kit type and flow cell format option.

a SBS: TruSeq Rapid SBS Kit v1 or HiSeq Rapid SBSKit v2

b Cluster Kit: TruSeq Rapid PE Cluster Kit v1, TruSeq Rapid SR Cluster Kit v1,

HiSeq Rapid PECluster Kit v2, or HiSeq Rapid SRCluster Kit v2

5 [Optional] Select the Use Existing Recipe checkbox to use a custom recipe. Otherwise,

allow the software to create the recipe from run parameters entered.

Sample Sheet Screen

Sample sheets are optional unless you are using BaseSpace to perform data analysis,

performing an indexing run, or planning to monitor demultiplexing performance using

Sequencing Analysis Viewer. For more information, see the Sequencing Analysis Viewer User

Guide (part # 15020619).

1 Select from the following options to specify the clustering method:

• Select On-Board Cluster Generation to perform clustering on-instrument.

• If clustering started on the cBot, select Template Hybridization on cBot.

2 Select Next.

3 In the Sample Sheet field, select Browse and navigate to the sample sheet location.

4 Select Next.

Reagents Screen

The Reagents screen records information about reagent kits used for the run. The reagent kit

ID (barcode number beginning with RGT) is used to determine reagent kit type and run

mode compatibility.

1 Scan or enter the SBS reagent kit ID.

2 For paired-end runs, scan or enter the reagent kit ID for the cluster kit.

3 Select the SBS reagent kit for the run:

• [For HiSeq Rapid SBS Kit v2] Select 500 Cycles for a 500 cycle kit. Cycles

remaining defaults to 525 cycles remaining.

• Select 200 Cycles for a 200 cycle kit. Cycles remaining defaults to 225.

• Select 50 Cycles for a 50 cycle kit. Cycles remaining defaults to 74.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 89