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Real-Time Analysis

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Part # 15035786 Rev. D

Color Matrix Correction

After registration and intensity extraction, Real-Time Analysis corrects for cross talk

between channels. Cross talk occurs when a cluster shows intensity in the C channel and

some intensity also shows in the A channel, for example. Using a 4x4 color matrix, Real-

Time Analysis generates matrix-corrected intensities with reduced or no cross talk, and

balances differences in overall intensity between color channels.

Empirical Phasing Correction

During the sequencing reaction, each DNA strand in a cluster extends by 1 base per cycle.

Phasing and prephasing occurs when a strand becomes out of phase with the current

incorporation cycle.

} Phasing occurs when a base falls behind.

} Prephasing occurs when a base jumps ahead.

Figure 50 Phasing and Prephasing

A Read with a base that is phasing

B Read with a base that is prephasing

Real-Time Analysis corrects the effects of phasing and prephasing using the empirical

phasing correction algorithm, which maximizes the data quality at every cycle throughout

the run.

Phasing and prephasing results are recorded in the file named EmpiricalPhasing_[lane]_

[read]_[tile].txt, which is located in the folder Data\Intensities\BaseCalls\Phasing.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 128