Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 85)

Pre-Run Volume Check

HiSeq 2500 System User Guide

Pre-Run Volume Check

From the Welcome screen, select Sequence | New Run. The Volume Check screen opens.

Volume Check Screen

1 When prompted by the software to perform a volume check, select Yes.

2 Place waste tubes 1, 2, 3, 6, 7, and 8 for the current flow cell in a 1 liter bottle filled with

deionized water. Placing the tubes in deionized water prevents damage to the reagent

pumps.

3 Load laboratory-grade water into all 8 SBS positions, 10 positions on the paired-end

rack, and the library position for the current flow cell.

4 Close the loading station.

5 Select the Water loaded and template loading station closed checkbox.

6 Select Next.

7 Make sure that a used rapid flow cell is loaded on the instrument. Enter the ID of the

used flow cell.

8 Select Next.

9 Select Pump to confirm flow.

10 Place tubes 4 and 5 into separate empty 15 ml conical tubes.

11 Select Next. The volume check begins.

When the volume check is complete, the expected volume is 9.5 ml ±10% for each tube.

12 Return all tubes to the waste bottle.

13 Select Next.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 85