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Perform a TruSeq v3 Run

Part # 15035786 Rev. D

Load Reagents for Read 2

After completion of Read 1 and any index reads, load paired-end reagents for Read 2

resynthesis and freshly prepared ICB for Read 2.

Load Paired-End Reagents

1 Record the weight of each reagent on the lab tracking form.

2 Make sure that the paired-end rack is not in use on the opposite flow cell for Read2

resynthesis, Index 1 (i7) Read preparation, or Index 2 (i5) Read preparation.

3 Raise the sippers for the paired-end reagent rack using the following motion:

a Pull the handle towards you and raise the handle.

b Release the handle into the slot on the top end of the groove. Make sure that the

handle rests securely in the slot.

4 Slide the reagent rack out of the reagent compartment using the rack handle.

5 Remove the caps from each reagent tube.

6 Place each of the reagent tubes onto the rack in the associated numbered positions.

Position

Reagent Description

RMR Resynthesis Mix

LMX2 Linearization Mix 2

BMX Blocking Mix

AMX2 Amplification Mix 2

APM2 AMX2 Premix

AT2 100% Formamide

HP7 or HP11 Read 2 Sequencing Primer

HP3 Denaturation Solution

HT2 Wash Buffer

Table 9 Paired-End Reagent Positions

NOTE

For dual-indexed paired-end runs, RMR is loaded with indexing reagents before starting

the run. For single-indexed or non-indexed runs, RMR is loaded with paired-end

reagents.

1 2 ... 71 72 73 74 75 76 77 78 79 80 81 ... 153 154

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Directed Electronics equalizer 2500 Guía de usuario Pagina 76