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Primer Rehybridization

HiSeq 2500 System User Guide

137

Primer Rehybridization

A rehybridization run repeats the sequencing primer hybridization step. If run metrics

indicate low cluster numbers, low cluster intensities, or other concerns, perform primer

rehybridization to rescue the flow cell. Primer rehybridization does not damage clusters on

the flow cell.

HiSeq v4 Flow Cell

All rehyb steps are performed on the HiSeq 2500. The kit includes primers for Read 1,

Index 1 Read, Index 2 Read for single-read flow cells, and Read 2.

Rehyb Kit Name Workflow Instructions

HiSeq Multi-Primer Rehyb Kit v4

Catalog # GD-403-4001

HiSeq Primer Rehybridization Guide (part #

15050105)

HiSeq v3 Flow Cell

Read 1 primer rehyb is performed on the cBot. The kit includes a cBot reagent plate

containing the Read 1 sequencing primer HP6. For Nextera libraries, use HP10 from the

TruSeq Dual Index Sequencing Primer Box.

Rehyb Kit Name Workflow Instructions

TruSeq v2 cBot Multi-Primer Rehyb Kit

Catalog # GD-304-2001

Read 1 Primer Rehybridization on the cBot (part #

15018149)

Rapid Flow Cell

All rehyb steps are performed on the HiSeq 2500. The kit includes primers for Read 1,

Index 1 Read, Index 2 Read for single-read flow cells, and Read 2.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 147