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Overview

Part # 15035786 Rev. D

Sequencing Consumables

Sequencing on the HiSeq 2500 requires reagents and other consumables provided in

Illumina kits. Required kits depend on the type of run to be performed.

} For HiSeq v4 high output runs, see HiSeq v4 Sequencing Consumables on page 18.

} For TruSeq v3 high output runs, see TruSeq v3 Sequencing Consumables on page 42.

} For Rapid Run, see Rapid Run Sequencing Consumables on page 70.

User-Supplied Consumables

Consumable Supplier Purpose

Tween 20, viscous liquid, 100 ml Sigma-Aldrich, catalog # P7949 Instrument maintenance

wash.

ProClin 300, 50 ml Sigma-Aldrich, catalog # 48912-U Instrument maintenance

wash.

Alcohol wipes,

70% Isopropyl

Ethanol, 70%

VWR, catalog # 95041-714

General lab supplier

Cleaning the flow cell and

flow cell stage.

Centrifuge tubes, 250 ml Corning, catalog # 430776 Instrument maintenance

wash and water wash.

Conical tubes, 15 ml Corning, catalog # 430052 Collecting and measuring

waste volumes.

Instrument maintenance

wash and water wash.

Conical tubes, 50ml,

self-standing (optional)

Corning, catalog#430921 Storing flow cells.

Carboy, at least 6 liters General lab supplier Maintenance wash solution.

Disposable gloves, powder-free General lab supplier General use.

Lab tissue, low-lint VWR, catalog # 21905-026 Cleaning the flow cell

holder.

Lens paper, 4 x 6 in VWR, catalog # 52846-001 Cleaning the flow cell.

Pipette tips, 200µl General lab supplier Splitting reagent kit

volumes.

Pipette tips, 1000µl General lab supplier Splitting reagent kit

volumes.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 24