
Perform a Rapid Run
86
Part # 15035786 Rev. D
Figure 37 Flow Cell Lever in Position 0
3 Put on a new pair of powder-free latex gloves.
4 If the flow cell from a previous run is present, remove it and set aside in a tube of
storage buffer or laboratory-grade water to keep it from drying out. It can be used to
confirm proper flow before loading the clustered flow cell.
5 Using an alcohol wipe or a lint-free tissue moistened with ethanol or isopropanol,
carefully wipe the surface of the flow cell holder until it is clean.
CAUTION
Do not allow alcohol to drip into the vacuum holes or around the manifolds. Use a low-lint
lab tissue to dry the stage, if necessary.
6 Visually inspect the flow cell holder to make sure that it is free of lint and the vacuum
holes are free of obstructions.
Figure 38 Vacuum Hole Locations
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