Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 150)

Index

140

Part # 15035786 Rev. D

vacuum hole locations 30, 54, 85

flow cell lever

position 0 30, 54, 85

position 1 31, 37, 55, 61

position 2 31, 37, 55, 61

flow cell loading

bubbles, leaks 33, 39, 57, 63

priming flow cell 31, 55

sequencing flow cell 37, 61

Flow Cell Set-up Screen 47, 77

Flow Cell Setup Screen 22

fluidics compartment 3

fluidics, troubleshooting 129, 131

folder locations, default settings 11

H

help

documentation 16

help, technical 143

HiSeq Control Software 7, 12

I

icons

errors and warnings 10

status alert 10

idling the instrument 108

images

monitoring 40, 64, 96

saving images samples 22, 47, 77

thumbnails 22, 47, 77, 122

indexed sequencing

reagents, loading 52

indexing scheme 24, 49

instrument health 11

instrument shutdown 109

instrument wash 100

intensity extraction 117

InterOp files 113, 122

L

LIMS server 11

loading reagents 27, 29, 66, 81

consumables 26

funnel caps 26

PW1 in position 2 26

SBS positions 26

weighing 26

locs files 121

log files, Real-Time Analysis 121

M

maintenance wash 8, 100

duration 106-107

maintenance wash solution

disposal 100

preparing 100

storing 100

modes

switching 106

N

no-calls 119

normal stop 133

O

online training 16

optics module 3

output files 121

base calls 113

cluster lcoations 113

output folder

default location 11

P

paired-end sequencing

reagents, loading 27, 29, 66

phasing, prephasing 118

Phred scale 119

priming

preparation 34, 58, 89

waste volume 34, 58, 89

Q

Q-scores 119

quality scores 119

binning 120

generating 115

R

reagent compartment 3-4

reagent positions

indexing 52

paired end 27, 29, 66

1 2 ... 145 146 147 148 149 150 151 152 153 154

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