Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 113)

Perform a Maintenance Wash

HiSeq 2500 System User Guide

103

17 [For Rapid Run mode] Place the ends of tubes 4 and 5 into an empty container. Place

the end of all of the other tubes into a bottle of clean water to prevent air from being

introduced into the syringe pumps.

18 Select Next to start the wash.

19 When the wash is complete, select Return to Start.

20 Measure the delivered volume.

NOTE

All bottles and tubes are filled to capacity to make sure that the sippers are rinsed. However,

the delivered volume for each position varies so the bottles and tubes contain different

volumes when the wash is complete.

Positions High Output Flow Cell

Delivered Volume

Rapid Flow Cell Delivered

Volume

8 SBS positions 82 ml 29ml

10 PE positions 76 ml 30ml

1 library position Empty 1.2 ml

All positions 19.75 ml per lane 30.1 ml per lane

21 Unwrap the waste tubes and return them to the waste container.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 113