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Perform a HiSeq v4 Run

Part # 15035786 Rev. D

Position Tubing and Start Prime

1 Remove the 8 waste tubes for the appropriate flow cell from the waste container.

Do not include the 8 tubes for the opposite flow cell or the tube for the condensation

pump.

Figure 15 Position Tubing

A Flow cell waste tubes for reagent positions 1–8

B Condensation pump tubing (do not remove)

2 Place waste tubing into an empty 15 ml tube, 1 waste tube per 15 ml tube. Priming

waste is collected and measured after the priming step.

3 Select Start Prime. The priming screen opens and the priming step begins. Monitor the

progress of the priming step from the priming screen.

4 When the priming step is complete, measure the collected waste and confirm that the

volume in each tube is 1.75 ml for a total of 14 ml. The total is calculated as follows:

• 250 µl for each SBS position except position 2 (250 x 7 = 1.75 ml)

• 1.75 ml for each lane (1.75 x 8 = 14 ml)

5 Record the results on the lab tracking form.

6 Return the waste tubing to the waste container before proceeding.

7 Select Next.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 44