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Sequencing Output Files

HiSeq 2500 System User Guide

121

Sequencing Output Files

File Type File Description, Location, and Name

Base call files Each tile analyzed is included in a base call file that contains the base

call and encoded quality score.

Data\Intensities\BaseCalls\L00[X]—Files are stored in per cycle

folders for each lane.

s_[Lane]_[Tile].bcl.gz, where lane is the single-digit lane number and

tile is the 4-digit tile number. Base call files are compressed using gzip

compression.

Cluster location files For each tile, 1 cluster location file contains the XY coordinates for

every cluster. Cluster location files are the result of template

generation.

Data\Intensities

Filter files The filter file specifies whether a cluster passed filters. Filter files are

generated at cycle 26 using 25 cycles of data.

Data\Intensities\BaseCalls\L00[X]—Files are stored in 1 folder for

each lane and tile.

s_[lane]_[tile].filter

InterOp files Binary reporting files used for Sequencing Analysis Viewer. InterOp

files are updated throughout the run.

InterOp folder

Log files Record events and are updated throughout the run.

Data\RTALogs

Offsets files Two offsets files are created for each run:

• offsets.txt—Contains tile offsets for every cycle and channel relative

to the template.

• SubTileOffsets.txt—Contains the measured shift for each quadrant

of each image relative to the frame of reference.

Data\Intensities\Offsets

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Directed Electronics equalizer 2500 Guía de usuario Pagina 131