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Perform a Rapid Run

Part # 15035786 Rev. D

Introduction

Rapid Run mode provides 2 options for the cluster generation step, either on the cBot or on

the HiSeq 2500. Clustering on the cBot allows 2 libraries, 1 each lane, on the 2-lane rapid

flow cell. After template hybridization and first extension on the cBot, the remainder of the

clustering process is performed on the HiSeq.

After reagent preparation, run setup steps include entering run parameters, loading and

priming reagents, loading the flow cell, and performing a fluidics check. If clustering is

performed on the HiSeq 2500, the step to prime reagents is omitted in the run setup steps.

Visit the HiSeq 2500 specifications page on the Illumina website for information about run

duration and other performance specifications.

Rapid Run Sequencing Consumables

Rapid Run Kit Name Description

HiSeq Rapid SBSKit v2

TruSeq Rapid SBS Kit (v1)

Contains SBSreagents used on the HiSeq 2500 and

funnel caps for SBSreagent bottles.

HiSeq Rapid PECluster Kit v2

HiSeq Rapid SRCluster Kit v2

TruSeq Rapid PE Cluster Kit (v1)

TruSeq Rapid SRCluster Kit (v1)

Contains clustering and indexing reagents used on the

HiSeq 2500 and a set of flow cell gaskets.

The PE versions of the cluster kits include paired-end

reagents used on the HiSeq 2500.

Reagent Preparation Steps

Before setting up the run, prepare SBS reagents, indexing reagents, and paired-end reagents.

} For SBS reagent preparation, see the appropriate guide:

• HiSeq Rapid SBSKit v2 Reagent Prep Guide (part # 15058772)

• TruSeq Rapid SBS Kit Reagent Prep Guide (200 Cycles) (part # 15036501)

• TruSeq Rapid SBS Kit Reagent Prep Guide (50 Cycles) (part # 15036502)

} For indexing and paired-end reagent preparation, see the appropriate guide:

• HiSeq Rapid Cluster Kit v2 ReagentPrep Guide (part # 15059131)

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Directed Electronics equalizer 2500 Guía de usuario Pagina 80