Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 40)

Perform a HiSeq v4 Run

Part # 15035786 Rev. D

Prime Reagents

Steps for priming reagents include cleaning the flow cell holder, loading a priming flow

cell, confirming proper flow, and then starting the prime.

Clean the Flow Cell Holder

1 Open the flow cell compartment door.

CAUTION

Do not place fluids on the flow cell compartment door or on the flow cell stage when the

door is open. Spills in this area can damage the instrument.

2 Make sure that the flow cell lever is in the OFF position.

Figure 10 Flow Cell Lever in Position 0

3 Put on a new pair of powder-free latex gloves.

4 If the flow cell from a previous run is present, remove it and set aside in a tube of

storage buffer or laboratory-grade water to keep it from drying out. It can be used to

confirm proper flow before loading the clustered flow cell.

5 Using an alcohol wipe or a lint-free tissue moistened with ethanol or isopropanol,

carefully wipe the surface of the flow cell holder until it is clean.

CAUTION

Do not allow alcohol to drip into the vacuum holes or around the manifolds. Use a low-lint

lab tissue to dry the stage, if necessary.

6 Visually inspect the flow cell holder to make sure that it is free of lint and the vacuum

holes are free of obstructions.

1 2 ... 35 36 37 38 39 40 41 42 43 44 45 ... 153 154

Sin comentarios

Directed Electronics equalizer 2500 Guía de usuario Pagina 40