Directed Electronics equalizer 2500 Guía de usuario Pagina 72

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Perform a TruSeq v3 Run
62
Part # 15035786 Rev. D
Figure 32 Flow Cell Positioned Against Top and Right Guide Pins
A Top guide pin
B Right guide pins
NOTE
Remove your hand from the flow cell before engaging the vacuum switch to prevent
possible alignment drift over time.
3 Slowly move the flow cell lever to position 1, which engages the vacuum and secures
the flow cell into position. When the flow cell lever is blinking green, the vacuum is
engaged. If the lever is not green, see Possible Run Setup Problems on page 129.
Figure 33 Flow Cell Lever in Position 1
4 Wait for about 5 seconds, and then slowly move the flow cell lever to position 2. When
the flow cell lever is solid green, the manifolds are in position and the flow cell is
ready for use.
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