Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 57)

Enter Run Parameters

HiSeq 2500 System User Guide

analysis, this field is optional.

2 Select Zip BCL files to reduce required storage space. If the run is connected to

BaseSpace, the Zip BCL files option is selected by default.

NOTE

The Bin Q-Scores setting is enabled by default to reduce required storage space. This setting

groups quality scores over a wider range of values without affecting accuracy or

performance.

3 Select from the following Save Auxiliary Files options:

• Save All Thumbnails—Saves all thumbnails images. A thumbnail is a sampling of

images from many tiles in each column of tiles, or swath, combined in 1 thumbnail

image.

• Save Tile Thumbnails—Saves tile thumbnails. Tile thumbnails represent a single

tile rather than a sampling of tiles in a swath.

4 Select Next.

Flow Cell Setup Screen

The Flow Cell Setup screen records information about the flow cell used for the run.

1 Scan the flow cell barcode or enter the flow cell ID (barcode number) of the flow cell to

be sequenced. The flow cell ID is used to determine flow cell type and reagent

compatibility.

2 Confirm that the flow cell type is HiSeq Flow Cell v3, which is automatically selected

based on the flow cell ID.

3 Enter an experiment name. The experiment name appears on each screen to help

identify the run in progress.

4 Enter a user name.

5 Select Next.

Advanced Screen

1 [Optional] Select the Confirm First Base checkbox.

A first base report is generated automatically for each run. Selecting this option opens

the first base report before proceeding with the run.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 57