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Real-Time Analysis

114

Part # 15035786 Rev. D

} Statistics files—For each cycle, 1 statistics file (*.stats) is produced. The statistics file

contains aggregate statistics for the cycle.

Primary output files are used for subsequent data analysis. Use bcl2fastq for

demultiplexing and conversion of .bcl files into FASTQ files, which can be used as input

for alignment. To convert data from the HiSeq, use bcl2fastq 1.8.4, or later.

Real-Time Analysis provides real-time metrics of run quality stored as InterOp files.

InterOp files are binary files containing tile, cycle, and read-level metrics, and are required

for viewing metrics in Sequencing Analysis Viewer. For viewing metrics generated by Real-

Time Analysis, use Sequencing Analysis Viewer v1.8.20 or later.

For more information, see Sequencing Output Files on page 121

Real-Time Analysis Error Handling

Real-Time Analysis keeps log files in the RTALogs folder. If an error occurs, it is recorded

in an error log file called *Error.txt.

NOTE

The software creates the error log file only if an error occurs.

Data Transfer

Throughout the run, Real-Time Analysis automatically copies data generated from raw

image files to the specified output folder location. If image analysis lags, Real-Time

Analysis stops processing and places the flow cell in a safe state. Processing resumes when

image data are available.

NOTE

If Real-Time Analysis stops functioning, processing is automatically resumed during the next

cycle at the appropriate point on the flow cell. Do not restart Real-Time Analysis manually.

If you are using BaseSpace, Illumina recommends a minimum network connection speed of

10 Mbps. For more information, see the HiSeq 2500, 1500, and 2000 Site Prep Guide (part #

15006407).

Data transfer is complete when a marker file called Basecalling_Netcopy_complete.txt is

generated. One of these files is generated for each read, and 1 is generated for the entire run.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 124