Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 112)

Post-Run Procedures

102

Part # 15035786 Rev. D

9 Wearing a new pair of gloves, apply light pressure to 1 side of the front gasket until the

other side lifts. Use tweezers to grasp and remove the gasket. Repeat to remove the rear

gasket.

Figure 49 Remove Used Manifold Gaskets

10 Position a new 10-port gasket in the front manifold and a new 8-port gasket in the rear

manifold.

11 Reload the flow cell.

12 Make sure that the Vacuum Engaged checkbox is selected on the Load Wash Flow Cell

screen.

13 Select Next.

14 Perform a fluidics check:

a Select solution 2 from the drop-down list. Accept the default pump values.

b Select Pump.

c Visually inspect the flow cell for bubbles passing through the lanes and leaks near

the manifolds. Extra bubbles are normal for the Tween 20 and ProClin 300 wash,

and do not affect delivered volume.

15 Remove the 8 waste tubes for the appropriate flow cell from the waste container. Do

not include the 8 tubes for the opposite flow cell, or the tubes from the condensation

pump.

16 [For high output modes] Bundle the 8 waste tubes with parafilm, making sure to keep

all of the tubes even. Place the bundled tube ends into a 250 ml bottle.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 112