Directed Electronics equalizer 2500 Guía de usuario descargar pdf (Pagina 94)

Perform a Rapid Run

Part # 15035786 Rev. D

positions:

• Paired-end run—Position 19

Non-indexed—Position 17

• Single-read run—Positions 10, 11, and 12

Non-indexed—Positions 16 and 17

13 Slide the PE rack into the reagent compartment, aligning the racks with the raised

guide on the floor of the compartment.

14 Lower the sippers into the sequencing reagent bottles as follows:

a Pull the sipper handle towards you and then lower the sipper handle.

b Visually inspect the sippers to make sure that they do not bend as they lower into

the funnel caps.

c Release the sipper handle into the slot on the bottom end of the groove.

Load Template

Load the library template for clustering on the instrument. For library preparation

instructions, see Denaturing and Diluting Libraries for the HiSeq and GAIIx (part # 15050107).

NOTE

If the cBot was used for clustering, place 2 Eppendorf tubes filled with 1 ml deionized water

into the loading station instead of using the following instructions.

1 Add 420 µl prepared library template in a 1.5 ml or 1.7 ml Eppendorf tube.

2 Load the template into the loading station, as follows:

a Lift the door of the loading station.

b Remove the Eppendorf tube containing water and replace it with the Eppendorf

tube containing 420 µl of prepared library template.

c Secure the lids under the bar behind the tubes to avoid interfering with the sippers.

1 2 ... 89 90 91 92 93 94 95 96 97 98 99 ... 153 154

Sin comentarios

Directed Electronics equalizer 2500 Guía de usuario Pagina 94