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Run Types for Rapid Run Chemistry

HiSeq 2500 System User Guide

Run Types for Rapid Run Chemistry

The following tables show types of sequencing runs and the number of possible cycles for

each read when using rapid run chemistry. Use this information as a reference when

setting up the run.

Run Type Read 1

Cycles

Index 1 (i7)

Read Cycles

Index 2 (i5)

Read Cycles

Cycles

Total

Cycles

Single-Read,

Non-Indexed

≤ 251 -- -- -- ≤ 251

Single-Read,

Single-Indexed

≤ 251 7 ¹

8 ²

-- -- ≤ 258 ¹

≤ 259 ²

Single-Read,

Dual-Indexed

≤ 251 8 8 -- ≤ 267

Paired-End,

Non-Indexed

≤ 251 -- -- ≤ 251 ≤ 502

Paired-End,

Single-Indexed

≤ 251 7 ¹

8 ²

-- ≤ 251 ≤ 509 ¹

≤ 510 ²

Paired-End,

Dual-Indexed

≤ 251 8 7 + 8 ³ ≤ 251 ≤ 525

Table 10 HiSeq Rapid SBSKit v2

Run Type Read 1

Cycles

Index 1 (i7)

Read Cycles

Index 2 (i5)

Read Cycles

Cycles

Total

Cycles

Single-Read,

Non-Indexed

≤ 101 -- -- -- ≤ 101

Single-Read,

Single-Indexed

≤ 101 7 ¹

8 ²

-- -- ≤ 108 ¹

≤ 109 ²

Single-Read,

Dual-Indexed

≤ 101 8 8 -- ≤ 117

Paired-End,

Non-Indexed

≤ 101 -- -- ≤ 101 ≤ 202

Paired-End,

Single-Indexed

≤ 101 7 ¹

8 ²

-- ≤ 101 ≤ 209 ¹

≤ 210 ²

Paired-End,

Dual-Indexed

≤ 101 8 7 + 8 ³ ≤ 101 ≤ 225

Table 11 TruSeq Rapid SBSKit (v1)

1 2 ... 78 79 80 81 82 83 84 85 86 87 88 ... 153 154

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