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Troubleshooting

136

Part # 15035786 Rev. D

Pause a Run

CAUTION

Do not pause a run during imaging. Use the Normal stop, end of cycle or end of lane feature,

to stop and resume a run.

Pause a run from the Run Overview screen. Pausing a run might be necessary to check run

components, such as reagent volumes, before proceeding with the run. Under normal

operation, pausing a run is not necessary.

1 From the Run Overview screen, select Pause. The pause menu opens.

Figure 55 Pause Options

2 Select Normal Pause.

3 Select Yes to confirm the pause command. The software completes the current

chemistry or imaging command and places the flow cell in a safe state.

4 Select Resume to resume the run.

Change Reagents During a Run

If you started the run with a partial volume of reagents, use the Change Reagents feature to

pause the run and replenish reagents.

1 From the Run Overview screen, select Pause. The pause menu opens.

2 Select Change Reagents.

3 Select Yes to confirm the pause command. The software completes the current

chemistry or imaging command, places the flow cell in a safe state, and opens the

Reagents screen.

4 On the Reagents screen, enter the following reagent parameters:

• The reagent kit ID for new reagents.

• The number of cycles the reagents are expected to last.

NOTE

The priming checkbox is disabled on the Change Reagents screen. Priming is not required.

5 Select Next to proceed to loading reagents.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 146