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Shut Down the Instrument

HiSeq 2500 System User Guide

109

Shut Down the Instrument

Shut down the instrument only if you do not plan to use it within the next 10 days or

more. If you plan to use the instrument within the next 10 days, see Idle the Instrument on

page 108.

Use the following procedure to prepare fluidics safely and shut down the system.

1 Perform a maintenance wash to flush the system. For more information, see Perform a

Maintenance Wash on page 100.

2 Remove the flow cell from the flow cell stage.

3 Using an alcohol wipe or a lint-free tissue moistened with ethanol or isopropanol,

carefully wipe the surface of the flow cell holder until it is clean.

CAUTION

Do not allow alcohol to drip into the vacuum holes or around the manifolds. Use a low-lint

lab tissue to dry the stage, if necessary.

4 [For high output modes] Load 10ml laboratory-grade water in each reagent position in

the reagent racks. Then lower the sippers.

5 [For Rapid Run mode] Load 10ml laboratory-grade water in each reagent position, and

1 ml laboratory-grade water in each loading station position. Then lower the sippers.

6 Turn off the instrument.

7 To restart the instrument, load water in all reagent positions, turn on the instrument,

and perform a water wash. For more information, see Perform a Water Wash on page

104.

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Directed Electronics equalizer 2500 Guía de usuario Pagina 119